Development of biosensors for the detection and quantification of small molecules and micro-organisms
16/11/2015 - 15/11/2019 (collaboration between ILVO and KU Leuven, Stella Givanoudi has a scholarship from ILVO)
Promotor: Prof. Patrick Wagner (KU Leuven - Laboratory for Soft Matter and Biophysics of the Faculty of Science), co-promotor: Prof. Marc Heyndrickx (ILVO - Technology and Food Science Unit) and dr. Johan Robbens (ILVO - Animal Sciences Unit)
Stella Givanoudi is a PhD student working at the Institute for Agricultural and Fisheries Research (ILVO) with a specialization in biosensors. Having graduated from the School of Biology of Aristotle University of Thessaloniki, she continued her studies as a postgraduate student at the School of Pharmacy of the same university. During her studies, she gained expertise in the fields of Molecular Biology, Biotechnology and Microbiology. Currently, she is a PhD student at Arenberg Doctoral School of KU Leuven and she is working at ILVO in the project “Development of biosensors for the detection and quantification of small molecules and micro-organisms”. This project is fully funded by ILVO and it will be carried out as a collaboration between ILVO and the Laboratory for Soft Matter and Biophysics of the Faculty of Science of KU Leuven under the supervision of Prof. Patrick Hermann Wagner. The project consists of two parts:
(A) The development of aptamer-based biosensors for biogenic amines.
Aptamers for the detection of biogenic amines will be developed using the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) process. Aptamers with high affinity and specificity will be selected through binding assays and indirect competition assays. Selected aptamers will be used for the functionalization of the biosensor’s surface and the detection of biogenic amines both in buffer liquids and in food samples.
(B) The development of surface imprinted polymer based biosensors for Campylobacter species.
Bacteria cells (Campylobacter jejuni and Campylobacter coli) will be imprinted in polyurethane layers and those surface imprinted polymers will be used as the biosensor’s surface for the detection of bacteria both in buffer liquids and in food samples.
Prof. Patrick Wagner (KULeuven)
Johan Robbens (ILVO)
Marc Heyndrickx (ILVO)
Katrien Broekaert (ILVO)